Confocal and Multiphoton Microscopes
Effective February 1, 2022 the optical microscopes in the Marley building, as well as the optical microscopes in Life Sciences North were administratively combined (with no equipment being moved) under Imaging Cores - Optical (iLab webpage). A much more functional website should come online later in the spring of 2022.
Zeiss LSM880 Inverted Confocal Microscope
- Contact person: Patty Jansma, 520-621-5097
- 34-channel detector for spectral information
- 7 Laser lines: 405, 458, 488, 514, 561, 594, 633nm
- Additional software modules for performing FRAP and control for the motorized stage to allow stitching together images from multiple fields of view into one large image (montage).
- Software modules for visiting multiple sites during a time lapse acquisition
- Environmental chambers suitable for live cell imaging at a variety of temperatures, humidity, and CO2 capabilities.
- Objective lenses (with matching DIC prisms) include:
- Plan-Apochromat 10x/0.45 (WD=2.0mm)
- Plan-Apochromat 20x/0.8 (WD=0.55mm)
- Plan-Apochromat 40x/1.3 Oil
- Plan-Apochromat 63x/1.4 Oil
- Plan-Apochromat 100x/1.4 Oil
- Additional lenses associated with the adjacent Zeiss LSM880 NLO multiphoton/confocal may be available for this instrument (please contact facility manager
Zeiss LSM880 NLO upright multiphoton/confocal microscope
- Contact person: Patty Jansma, 520-621-5097
- 34-channel detector for spectral information
- 7 Laser lines: 405, 458, 488, 514, 561, 594, 633nm plus an additional software tunable Spectra Physics Mai Tai HP DeepSee laser (690-1040 nm)
- Additional software modules for controlled region-of-interest scanning, deconvolution, physiology experiments, performing FRAP& FCS and control for the motorized stage to allow stitching together images from multiple fields of view into on larger image (montage).
- Software modules for visiting multiple sites during a time lapse acquisition
- Environmental chambers suitable for live cell imaging at a variety of temperatures.
- Objective lenses (with matching DIC prisms) include:
- N-Achroplan 5x/0.15 (WD=12.0mm)
- Plan-Apochromat 10x/0.45 (WD=2.0mm)
- Plan-Apochromat 20x/0.8 (WD=0.55mm)
- W Plan-Apochromat 20x/1.0 Water
- C-ACHROPLAN 32x/0.85 W Korr water
- EC Plan-Apochromat 40x/1.3 Oil
- C Planapo 40x/1.3 oil
- LD C-Apochromat 40x/1.3 Oil
- Plan-Apochromat 63x/1.4 Oil
- W Plan-Apo 63x/1.0 Water (WD=2.1mm)
- Plan-Apochromat 100x/1.4 Oil
- Additional lenses associated with the adjacent Zeiss LSM880 NLO multiphoton/confocal may be available for this instrument (please contact facility manager)
Image Processing workstation
High end workstation for image processing
- Contact person: Patty Jansma, 520-621-5097
- For additional processing of Airyscan images
- Software includes Zeiss Zen Blue and Black, as well as FIJI (ImageJ).
Stereo Microscope
Zeiss Axiozoom 16 fluorescent stereo microscope
- Contact person: Patty Jansma, 520-621-5097
- Equipped with a Hamamatsu Flash 4.0 camera
- Computer has Zeiss Zen Blue software for combining the grayscale images into color overlays.
- Fluorescent filters include UV (DAPI), Blue (GFP, FITC, Alexa488) and Green (RFP, Alexa 555).
- The magnification ranges from 7-112x.
- Includes a fiber optic light to enable brightfield images.
Microwave processor
Pelco Biowave Pro microwave tissue processor
- Programmable microwave tissue processor, with continuous power settings from 100-750 Watts, load cooling to maintain consistent temperatures, built-in fume extraction and built-in vacuum.
- Contact person: Patty Jansma, 520-621-5097
- Useful for:
- Improved tissue fixation
- Improved retention of fluorescence after fixation
- More rapid tissue processing for immunocytochemistry, electron microscopy, and decalcification