Marley: Light Microscopy

Zeiss LSM880 Inverted Confocal Microscope

• Contact person: Patty Jansma, 520-621-5097
• 34-channel detector for spectral information
• 7 Laser lines: 405, 458, 488, 514, 561, 594, 633nm
• Additional software modules for performing FRAP and control for the motorized stage to allow stitching together images from multiple fields of view into one large image (montage).
• Software modules for visiting multiple sites during a time lapse acquisition
• Environmental chambers suitable for live cell imaging at a variety of temperatures, humidity, and CO2 capabilities.
• Objective lenses (with matching DIC prisms) include:
  • Plan-Apochromat 10x/0.45 (WD=2.0mm)
  • Plan-Apochromat 20x/0.8 (WD=0.55mm)
  • Plan-Apochromat 40x/1.3 Oil
  • Plan-Apochromat 63x/1.4 Oil
  • Plan-Apochromat 100x/1.4 Oil
  • Additional lenses associated with the adjacent Zeiss LSM880 NLO multiphoton/confocal may be available for this instrument (please contact facility manager

Schedule User Time

Zeiss LSM880 NLO upright multiphoton/confocal microscope

• Contact person: Patty Jansma, 520-621-5097
• 34-channel detector for spectral information
• 7 Laser lines: 405, 458, 488, 514, 561, 594, 633nm plus an additional software tunable Spectra Physics Mai Tai HP DeepSee laser (690-1040 nm)
• Additional software modules for controlled region-of-interest scanning, deconvolution, physiology experiments, performing FRAP& FCS and control for the motorized stage to allow stitching together images from multiple fields of view into on larger image (montage).
• Software modules for visiting multiple sites during a time lapse acquisition
• Environmental chambers suitable for live cell imaging at a variety of temperatures.
• Objective lenses (with matching DIC prisms) include:
  • N-Achroplan 5x/0.15 (WD=12.0mm)
  • Plan-Apochromat 10x/0.45 (WD=2.0mm)
  • Plan-Apochromat 20x/0.8 (WD=0.55mm)
  • W Plan-Apochromat 20x/1.0 Water
  • C-ACHROPLAN 32x/0.85 W Korr water
  • EC Plan-Apochromat 40x/1.3 Oil
  • C Planapo 40x/1.3 oil
  • LD C-Apochromat 40x/1.3 Oil
  • Plan-Apochromat 63x/1.4 Oil
  • W Plan-Apo 63x/1.0 Water (WD=2.1mm)
  • Plan-Apochromat 100x/1.4 Oil
  • Additional lenses associated with the adjacent Zeiss LSM880 NLO multiphoton/confocal may be available for this instrument (please contact facility manager)

Schedule User Time

High end workstation for image processing

• Contact person: Patty Jansma, 520-621-5097
• For additional processing of Airyscan images
• Software includes Zeiss Zen Blue and Black, as well as FIJI (ImageJ).

Schedule User Time

Zeiss Axiozoom 16 fluorescent stereo microscope

• Contact person: Patty Jansma, 520-621-5097
• Equipped with a Hamamatsu Flash 4.0 camera
• Computer has Zeiss Zen Blue software for combining the grayscale images into color overlays.
• Fluorescent filters include UV (DAPI), Blue (GFP, FITC, Alexa488) and Green (RFP, Alexa 555).
• The magnification ranges from 7-112x.
• Includes a fiber optic light to enable brightfield images.

Pelco Biowave Pro microwave tissue processor

• Programmable microwave tissue processor, with continuous power settings from 100-750 Watts, load cooling to maintain consistent temperatures, built-in fume extraction and built-in vacuum.
• Contact person: Patty Jansma, 520-621-5097
• Useful for:
• Improved tissue fixation
• Improved retention of fluorescence after fixation
• More rapid tissue processing for immunocytochemistry, electron microscopy, and decalcification