Confocal and Multiphoton Microscopes
Zeiss LSM880 Inverted Confocal Microscope
- Contact person: Patty Jansma, 520-621-5097
- 34-channel detector for spectral information
- 7 Laser lines: 405, 458, 488, 514, 561, 594, 633nm
- Additional software modules for performing FRAP and control for the motorized stage to allow stitching together images from multiple fields of view into one large image (montage).
- Software modules for visiting multiple sites during a time lapse acquisition
- Environmental chambers suitable for live cell imaging at a variety of temperatures, humidity, and CO2 capabilities.
- Objective lenses (with matching DIC prisms) include:
- Plan-Apochromat 10x/0.45 (WD=2.0mm)
- Plan-Apochromat 20x/0.8 (WD=0.55mm)
- Plan-Apochromat 40x/1.3 Oil
- Plan-Apochromat 63x/1.4 Oil
- Plan-Apochromat 100x/1.4 Oil
- Additional lenses associated with the adjacent Zeiss LSM880 NLO multiphoton/confocal may be available for this instrument (please contact facility manager
Zeiss LSM880 NLO upright multiphoton/confocal microscope
- Contact person: Patty Jansma, 520-621-5097
- 34-channel detector for spectral information
- 7 Laser lines: 405, 458, 488, 514, 561, 594, 633nm plus an additional software tunable Spectra Physics Mai Tai HP DeepSee laser (690-1040 nm)
- Additional software modules for controlled region-of-interest scanning, deconvolution, physiology experiments, performing FRAP& FCS and control for the motorized stage to allow stitching together images from multiple fields of view into on larger image (montage).
- Software modules for visiting multiple sites during a time lapse acquisition
- Environmental chambers suitable for live cell imaging at a variety of temperatures.
- Objective lenses (with matching DIC prisms) include:
- N-Achroplan 5x/0.15 (WD=12.0mm)
- Plan-Apochromat 10x/0.45 (WD=2.0mm)
- Plan-Apochromat 20x/0.8 (WD=0.55mm)
- W Plan-Apochromat 20x/1.0 Water
- C-ACHROPLAN 32x/0.85 W Korr water
- EC Plan-Apochromat 40x/1.3 Oil
- C Planapo 40x/1.3 oil
- LD C-Apochromat 40x/1.3 Oil
- Plan-Apochromat 63x/1.4 Oil
- W Plan-Apo 63x/1.0 Water (WD=2.1mm)
- Plan-Apochromat 100x/1.4 Oil
- Additional lenses associated with the adjacent Zeiss LSM880 NLO multiphoton/confocal may be available for this instrument (please contact facility manager)
Image Processing workstation
High end workstation for image processing
- Contact person: Patty Jansma, 520-621-5097
- For additional processing of Airyscan images
- Software includes Zeiss Zen Blue and Black, as well as FIJI (ImageJ).
Stereo Microscope
Zeiss Axiozoom 16 fluorescent stereo microscope
- Contact person: Patty Jansma, 520-621-5097
- Equipped with a Hamamatsu Flash 4.0 camera
- Computer has Zeiss Zen Blue software for combining the grayscale images into color overlays.
- Fluorescent filters include UV (DAPI), Blue (GFP, FITC, Alexa488) and Green (RFP, Alexa 555).
- The magnification ranges from 7-112x.
- Includes a fiber optic light to enable brightfield images.
Microwave processor
Pelco Biowave Pro microwave tissue processor
- Programmable microwave tissue processor, with continuous power settings from 100-750 Watts, load cooling to maintain consistent temperatures, built-in fume extraction and built-in vacuum.
- Contact person: Patty Jansma, 520-621-5097
- Useful for:
- Improved tissue fixation
- Improved retention of fluorescence after fixation
- More rapid tissue processing for immunocytochemistry, electron microscopy, and decalcification